AMDCC :: Protocols

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Diabetes Induction
Low-Dose Streptozotocin Induction Protocol (mouse)
Low-dose STZ + high-fat diet (mouse)

Dissection
Animal SPT Catheter Implantation
Isolation of rodent eyes for analysis of diabetic retinopathy
Removal and Preservation of the Urinary Bladder

Histology
En face preparation and quantification of aortic surface area covered by atherosclerotic lesions
Mouse dissection and preparation of heart (aortic sinus) and brachiocephalic artery for lesion quantitation by sectioning

Phenotype Assay
Aortic Banding in Mice
Atherosclerotic Morphometry in Pigs
Cardiac AMPK Assay
CATALASE
Conscious Cystometry Bladder Function Testing
Creatinine Clearance by HPLC
Creatinine Companion Protocol Assay (Exocell)
Determination of Glomerular Filtration Rate in Conscious Mice using FITC-inulin
Echocardiography: Mouse
Evaluation of Mitochondrial Function
Frequently sampled intravenous glucose tolerance test
Glucose "Clamp" for measurement of whole-body insulin stimulated glucose disposal
Glucose uptake in isolated cardiomyocytes.
Intraperitoneal Glucose Tolerance Testing (IPGTT)
Isoproterenol
Lipid Profiles in Mice
Lipoprotein separation by FPLC
Measurement of Left Ventricular Hemodynamic Parameters in Intact Mice
Measurement of Left Ventricular Performance in Langendorff Perfused Mouse Hearts
Mesangial Index Quantification
Morphometry: Mouse
Murine Microalbuminuria ELISA (Albuwell M kit)
NADH Oxidase Activity
Nerve Conduction Velocity Tests
Neuropathy Phentoyping Protocols
Phenotypic Characterization of the Working Heart
Plantar Analgesia Test for Hind Paw and Tail
Plasma Insulin (LINCO ELISA)
Podocyte Count and Density Analysis
Quantification of atherosclerosis at the aortic sinus
Serum, Blood and Body Weight Measurements in Pigs
Tail Flick
Thiobarbituric acid reactive substances
TRAP
Ultra Sensitive Rat Insulin ELISA Kit
Ultracentrifugal separation of HDL alone and calculation of non-HDL
Ultracentrifugal separation of VLDL, LDL and HDL
Western Analysis used in Oxidative Stress Protocols

Technique
Determination of Podocyte Number and Density in Rodent Glomeruli
Vascular perfusion of mice
WT-1 Staining Protocol for Podocytes